Dose-response analysis was developed by the field of toxicology to identify the concentration at which a biological assay responds to chemical exposure. Dose response analysis require doses or exposure levels span a wide range to perform curve fitting. Two dose types are currently accepted:

• Repeated dosing: this experimental design includes a control group (dose = 0) and at least three different dose groups, typically with the same number of replicates in each group.
• Continuous exposure: this data is usually from observational exposomics studies with doses/exposures measured from individuals with different exposure levels;