MetaboAnalyst

Lipid Class Enrichment

Tests whether lipid categories, lipid classes or lipid pathways are over-represented among your significant lipids (a list), or associated with the outcome in a table of lipid concentrations.

Lipid names are parsed (rgoslin, Shorthand 2020) and matched at their own level against a focused database of 190,104 entries (the MetaboAnalyst lipid database, LIPID MAPS and HMDB): 189,764 lipids in LIPID MAPS categories and main classes, and 340 compounds of the lipid pathways without a lipid category, which count only in the Lipid pathways library. A sum-composition name such as PC 34:1 matches the species PC 34:1, never one of its molecular species; a structure without an entry of its own is counted at its species.

Lipid list
Concentration table

Enter the significant lipids, one per line:

Lipid names in shorthand notation (PC 34:1, PC(16:0/18:1(9Z)), SM(d18:1/16:0)) or HMDB IDs.

ID type:

Example: An illustrative list, not from a study: 15 common plasma lipid species (phosphatidylcholines, sphingomyelins, ceramides, triacylglycerols, cholesteryl esters); shorthand names.

Upload a table of lipid concentrations (two groups or a continuous outcome):

Lipids named in shorthand notation or identified by HMDB IDs.

Group label:	Discrete (two groups) Continuous
ID type:
Data format:
Data file:	Choose

Example data (multiple_sclerosis_test.csv): Serum lipids of 33 patients with multiple sclerosis, 13 with benign MS and 20 with secondary progressive MS (SPMS): 119 lipids in shorthand notation, samples in columns. Metabolomics Workbench study ST000688.