MetaboAnalyst

Microbial Metabolite Enrichment

Tests whether metabolites made or transformed by gut microbes are over-represented among your significant compounds (a list), or associated with the outcome in a concentration table: by microbial function (short-chain fatty acids, bile acids, indoles, TMAO, ...), chemical class, producing genus or transformed substrate.

Names are matched against a focused database of 5,645 compounds: 3,675 microbial metabolites (MiMeDB, Exposome-Explorer) and 1,970 compounds known only from KEGG gut bacterial pathways, which count only in the Gut bacterial pathways library. A name that is not found is not in these sources under that name.

Compound list
Concentration table

Enter the significant compounds, one per line:

Compound names, HMDB IDs, KEGG IDs or PubChem CIDs.

ID type:

Example: An illustrative list, not from a study: 18 well-known gut microbial metabolites (short-chain fatty acids, secondary bile acids, indoles, uraemic solutes, polyphenol metabolites); compound names.

Upload a table of compound concentrations (two groups or a continuous outcome):

Compounds named or identified by HMDB IDs, KEGG IDs or PubChem CIDs.

Group label:	Discrete (two groups) Continuous
ID type:
Data format:
Data file:	Choose

Example data (micro_t1d_fecal.csv): Faecal metabolite abundances of 63 children with new-onset type 1 diabetes (T1D) and 76 healthy controls (NC): 331 compounds identified by KEGG IDs, samples in columns. Yuan et al., Nat Commun 2022;13:6356, as curated for the MicrobiomeAnalyst example data.