Spatial Lipid Annotation

A pixel x m/z table from MALDI or DESI imaging with coordinates and regions: m/z are matched to lipid species (sum composition, LIPID MAPS shorthand), then summarised as lipid-class tissue maps, class share and statistics per region, and chain length / unsaturation profiles.

Two files: intensity table and spatial metadata (.csv / .txt)
Intensity table: Choose
Spatial metadata: Choose
ROI column: auto = first of ROI / group / region / cluster / ssc
Ion mode:
Ion source:
MALDI: [M+H]+, [M+Na]+, [M+K]+ or [M-H]-, [M+Cl]-; DESI adds [M+NH4]+ or [M+HCOO]-, [M+CH3COO]-
Mass tolerance (ppm):
editable; FTICR / Orbitrap 3-5, TOF 10-20
Try our example data
Data Platform Description
Intensity table
Spatial metadata
METASPACE annotations		 MALDI-FTICR, DHB, positive Mouse brain lipids (SMA). Coronal mouse brain section from the SMA study (Vicari et al., Nat Biotechnol 2024; section V11L12-038 A1; Mendeley Data 10.17632/w7nw4km7xd.1): 2,598 pixels with an anatomical label (9 regions, from the Visium layer of the same section) x the 600 most variable m/z of the run; pixels registered to the Visium micrometre coordinates. About 110 of the 600 m/z match a lipid ion at 5 ppm, 62 of them as a usual ion of their class; the others have no lipid match in the library (metabolites, matrix and isotope peaks, or lipids and ions not covered). The main phosphatidylcholine ions of brain (PC 32:0, 34:1, 36:1, 38:6) are not among the 600 m/z, so the class results illustrate the workflow rather than the lipid composition of the brain. The METASPACE annotations of the same section (FDR ≤ 20%) are given for comparison. Settings: positive mode, MALDI, 5 ppm.